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- Additional Genomics Services
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In addition to Next-Generation Sequencing, Sanger sequencing, and array platforms and analyses, the OGI Genomics Core also offers the following services:
Next-Generation Sequencing Sample Preparation
SureSelect Targeted Enrichment, Agilent Technologies, Inc.
- Exome
- CUSTOM panels/designs
Whole Genome, Illumina, Inc.
- TruSeq (large genomes)
- NEXTERA (small genomes)
Transcriptome
Genomic DNA Blood Extraction
- Kit: Gentra PureGene Blood Kit
- Concentration/Purity assessment: NanoDrop 8000, Thermo Scientific, Inc.
- Certification: CLIA
Quantitative and Qualitative Biopolymer Analyses
NanoDrop 8000 (UV-Vis Spectrophotometer)
- dsDNA
- ssDNA
- RNA
- Protein
Up to eight simultaneous analyses
- Sample concentration
- Sample purity A260/A280 (protein) and A260/A230 (organics)
- Fluorescent labeling efficiency
Qubit Fluorometer, Life Technologies, Inc.
dsDNA
2200 TapeStation, Agilent Technologies, Inc.
DNA
Ultrasonication (i.e. “Mechanical Sheering”)
E220 Multisample Ultrasonicator, Covaris, Inc.
User-tuned for ultrasonication (i.e. “sheering”) of biological samples, including:
- gDNA
- RNA
- Tissue
- FFPE
Can sequentially sheer between 1 – 24/48 samples
Automation
Bravo NGS workstation, Agilent Technologies, Inc.
Automated preparation of multiplexed, selective capture libraries for NGS
For questions regarding the above equipment or services, please contact Caitlin Finn, OGI’s Genomics Core Lab Manager, at OGICore@meei.harvard.edu.