Additional Genomics Services
In addition to Next-Generation Sequencing, Sanger sequencing, and array platforms and analyses, the OGI Genomics Core also offers the following services:
Next-Generation Sequencing Sample Preparation
SureSelect Targeted Enrichment, Agilent Technologies, Inc.
- CUSTOM panels/designs
Whole Genome, Illumina, Inc.
- TruSeq (large genomes)
- NEXTERA (small genomes)
Genomic DNA Blood Extraction
- Kit: Gentra PureGene Blood Kit
- Concentration/Purity assessment: NanoDrop 8000, Thermo Scientific, Inc.
- Certification: CLIA
Quantitative and Qualitative Biopolymer Analyses
NanoDrop 8000 (UV-Vis Spectrophotometer)
Up to eight simultaneous analyses
- Sample concentration
- Sample purity A260/A280 (protein) and A260/A230 (organics)
- Fluorescent labeling efficiency
Qubit Fluorometer, Life Technologies, Inc.
2200 TapeStation, Agilent Technologies, Inc.
Ultrasonication (i.e. “Mechanical Sheering”)
E220 Multisample Ultrasonicator, Covaris, Inc.
User-tuned for ultrasonication (i.e. “sheering”) of biological samples, including:
Can sequentially sheer between 1 – 24/48 samples
Bravo NGS workstation, Agilent Technologies, Inc.
Automated preparation of multiplexed, selective capture libraries for NGS
For questions regarding the above equipment or services, please contact Caitlin Finn, OGI’s Genomics Core Lab Manager, at OGICore@meei.harvard.edu.