In addition to Next-Generation Sequencing, Sanger sequencing, and array platforms and analyses, the OGI Genomics Core also offers the following services:

 

Next-Generation Sequencing Sample Preparation

 

SureSelect Targeted Enrichment, Agilent Technologies, Inc.

  • Exome
  • CUSTOM panels/designs

Whole Genome, Illumina, Inc.

  • TruSeq (large genomes)
  • NEXTERA (small genomes)

Transcriptome

 

Genomic DNA Blood Extraction

 

 

 

Quantitative and Qualitative Biopolymer Analyses

 

 

NanoDrop 8000 (UV-Vis Spectrophotometer)

  • dsDNA
  • ssDNA
  • RNA
  • Protein

Up to eight simultaneous analyses

  • Sample concentration
  • Sample purity  A260/A280 (protein) and A260/A230 (organics)
  • Fluorescent labeling efficiency

 

Qubit Fluorometer, Life Technologies, Inc.

dsDNA

 

2200 TapeStation, Agilent Technologies, Inc.

DNA

RNA

 

Ultrasonication (i.e. “Mechanical Sheering”)

E220 Multisample Ultrasonicator, Covaris, Inc.

User-tuned for ultrasonication (i.e. “sheering”) of biological samples, including:

  • gDNA
  • RNA
  • Tissue
  • FFPE

Can sequentially sheer between 1 – 24/48 samples

 

Automation

Bravo NGS workstation, Agilent Technologies, Inc.

Automated preparation of multiplexed, selective capture libraries for NGS

 

For questions regarding the above equipment or services, please contact Caitlin Finn, OGI’s Genomics Core Lab Manager, at OGICore@meei.harvard.edu.