Open Positions

Index of positions

Position #1158: Research Technologist
Position #1100: Post Doctoral Researcher





Position #1158: Research Technologist


Description:

REPORTS TO:  Dr. Kinga Bujakowska                                    

POSITION SUMMARY:

We seek a motivated Research Technologist to join a research lab in the Ocular Genomics Institute (OGI) at the Massachusetts Eye and Ear in Boston, MA.  The successful candidate will carry out molecular biology, cell biology and genetic research studying inherited retinal degenerations under the supervision of Dr. Bujakowska. The ideal candidate will have molecular biology background and experience in RNA and DNA isolation, PCR, cDNA synthesis, cloning into plasmid vectors and next-generation sequencing basic knowledge. The candidate will work with a large DNA collection, which requires high level of meticulousness. Experience in working with animal and cell-based models would be an asset, as the candidate may perform some experiments in zebrafish or mice. The candidate must be able to execute the experiments with precision and understanding of theory behind methods used. The candidate must be reliable, organized, independent, and able to multi-task.  Please send you resume to kinga_bujakowska@meei.harvard.edu.

ESSENTIAL FUNCTIONS:

  1. Carries out a variety of research experiments using techniques that include but are not limited to: RNA and DNA isolation; cDNA synthesis; PCR; cloning into plasmid and virus vectors; primer design; immunohistochemistry.
  2. Maintains and performs experiments with human and animal cell lines, including but are not limited to: Isolation of primary cells; transfection; transduction and in vitro differentiations.
  3. Uses computer software or programs to perform primer design, sequence analyses, construction of plasmids, ect.
  4. Records, graphically displays, and presents pertinent information obtained from research activities through preparation of charts, illustrations, slides, statistical summaries graphs, and written/narrative reports. Provides information that will typically be used as a basis for further investigation and/or for inclusion in publication(s).
  5. Operates and maintains a variety of moderately complex lab equipment such as scales, centrifuges, thermal cyclers, and other scientific apparatus and equipment related to research activities.  Performs basic maintenance on laboratory equipment (cleans, disassembles, stores, replaces worn parts) and reports equipment requiring more advanced repair.
  6. Helps monitor supply inventory, and order supplies for general lab use.

ADDITIONAL SKILLS

  1. The candidate may work with zebrafish or mouse models, including: caring for and maintaining colonies, animal surgery and tissue processing.

EDUCATION AND EXPERIENCE:

Bachelors/Masters degree in biological sciences or a related field with 5+ years of experience working in a research lab.  The ideal candidate will have experience with molecular biology techniques such as PCR, DNA/RNA extraction, cloning

WORKING CONDITIONS:

Laboratory environment.

PHYSICAL REQUIREMENTS:

No special requirements.

Interested candidates should send resume and 3 references to Kinga_Bujakowska@meei.harvard.edu

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Position #1100: Post Doctoral Researcher


Description:

We are seeking a talented and motivated postdoctoral fellow to join our team in the Ocular Genomics Institute at Massachusetts Eye and Ear and Harvard Medical School to investigate the application of AAV-mediated gene therapy and CRISPR/Cas9 genome editing technology for the treatment of human genetic diseases in the eye.  The eye has been at the forefront of translational gene therapy due to the anatomic accessibility and well-studied immunologic privilege. The first FDA-approved gene therapy for inherited disease is for one form of an inherited retinal degenerative diseases (IRDs). The postdoc will conduct cutting-edge researches to develop therapeutic genome editing approaches for the treatment of several forms of IRDs. The projects are conducted in collaboration with the world’s leading experts in CRISPR field in Harvard Medical School and the Broad Institute. This position is available immediately.

Qualified candidates must have a PhD degree in Biology, Genetics, or Biomedical Sciences and have strong molecular/cellular biologic skills.  A strong publication record is preferred. Work experience with genetically modified animal models, CRISPR technology, iPS cells or retinal research is preferred but not absolutely required.  We expect you to be flexible, open to new ideas, ambitious, an out-of-the-box thinker, and result oriented. Attitude is as important as aptitude. Applicants must be proficient in both written and spoken English.  Applicants must have excellent communication and collaboration skills, and be able to work independently as well as in a group environment.

Interested candidates are asked to clearly indicate, in a one to two page cover letter, their lab expertise, especially in genetic and genomics, genome engineering, iPSC or gene therapy, and how you have used your skills and knowledge to shape your science. Candidates should also include a three-four sentence of description on how you would propose to apply CRISPR/Cas9 technology for monogenic inherited diseases.  Please send your CV, cover letter, and contact information for 3 references to:

Dr. Qin Liu, MD, PhD

qin_liu@meei.harvard.edu

Requirements:

Qualified candidates must have a PhD degree in Biology, Genetics, or Biomedical Sciences and have strong molecular/cellular biologic skills. A strong publication record is preferred. Work experience with genetically modified animal models, CRISPR technology, iPS cells or retinal research is preferred but not absolutely required. We expect you to be flexible, open to new ideas, ambitious, an out-of-the-box thinker, and result oriented. Attitude is as important as aptitude. Applicants must be proficient in both written and spoken English. Applicants must have excellent communication and collaboration skills, and be able to work independently as well as in a group environment.

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