Functional Genomics (Zebrafish) Core
Services provided by the core:
- Housing and general care of zebrafish (feeding, breeding, health monitoring, system maintenance)
- Training in zebrafish handling and husbandry
- Assistance with zebrafish protocol planning and preparation
- Propagation of wild-type zebrafish stocks for embryo production
- Importing and maintenance of transgenic or mutant lines
- Fertilized wild type embryos for injection
- Microinjection of zebrafish embryos
- Morpholino-based gene knockdown strategies (including assistance with design of morpholinos for specific genes, evaluation of knockdown efficiency on mRNA level, and generation of constructs to obtain wild-type and mutant mRNA for the human orthologues for complementation assays)
- CRISPR/Cas9 genome editing and establishment of edited lines (including assistance with design and generation of guide RNAs to target specific genes, and evaluation of CRISPR/Cas9 efficiency in vivo)
- Molecular biology and histology (in situ hybridization, histological, or immunohistological analyses)
- Vision assessment (visual motor response)
- Intraocular pressure measurement
Contacts:
Kinga Bujakowska (Kinga_Bujakowska@meei.harvard.edu)
Anna Larson (Anna_Larson@meei.harvard.edu)