Sequencing Services:
Capillary-based Sanger sequencing:
Applied Biosystems SeqStudio Flex 24 genetic analyzer for Sanger sequencing and fragment analysis with a 24-capillary configuration, four-plate capacity with continuous plate loading for mid-throughput capacity.
| Service | Qty | Unit | MGB Pricing | External Academic Pricing |
| Sanger Sequencing Individual sample cost 1-94 samples | 1 | Sample | $3.99 | $5.75 |
| Sanger Sequencing Bulk discount; per plate of 95 samples | 1 | Plate (95 samples) | $320.00 | $460.80 |
| Sanger Sequencing Difficult template Individual sample cost 1-94 samples | 1 | Sample | $4.85 | $6.98 |
| Sanger Sequencing Difficult template Bulk discount; per plate of 95 samples | 1 | Plate (95 samples) | $390.00 | $561.60 |
/table>
NGS Sequencing Services:
The Genomics core provides a wide range of next generation sequencing services. We offer amplicon sequencing, whole plasmid sequencing, whole exome sequencing, selective exon capture and next generation sequencing NGS) for ocular disease genes RetNeT, glaucoma, optic neuropathy), whole genome sequencing, and RNA sequencing for transcriptome analyses. We can also assist you with your custom sequencing project, please contact us for a consultation or request a quote.
Request a Quote:
To request a quote, please email ogicore@meei.harvard.edu with the following information:
Investigator Name
Phone Number
Email Address
Request Information
*Request Information should include as much detail as possible about your project requirements. For guidance, please see the pages under your project type for more information.
Sequencing Only Projects Includes library quantification, denaturation, sequence data generation and delivery. Does not include library preparation and pooling.
Library Preparation and Sequencing Projects Includes sample QC upon arrival, library preparation for NGS, library QC and normalization, plus the above items.
Data Analysis using Illumina Dragen v4 available. Available analysis pipelines include Whole genome, DNA Amplicon, RNA and Single-Cell RNA, RNA Amplicon, Methylation, Metagenomics, COVID and COVIDSeq, TSO 500 ctDNA, Imputation, and ScATAC-Seq.
We strongly recommend a joint consultation when planning and requesting new sequencing projects. Custom Analysis upon request through the MEEI Bioinformatics Center (MBC) (Ocular Genomics Institute (harvard.edu). Please email Senior Bioinformaticist Sudeep Mehrotra (Sudeep_mehrota@meei.harvard.edu) for more information.
Sequencing Only:
Includes library quantification, denaturation, sequence data generation and delivery. Does not include library preparation and pooling. Illumina sequencing through the OGI is only available by flow cell and not on a per read basis. If you wish to “share” flow cells, this may affect your wait time as samples will be combined based on read-length, index compatibility and desired coverage however the wait is usually within 2-3 weeks.
NovaSeq 6000
| Flow Cell | Single Reads | Paired-End Reads |
| SP | 650-800M | 1.3-1.6B |
| S1 | 1.3-1.6B | 2.6-3.2B |
| S2 | 3.3-4.1B | 6.6-8.2B |
| S4 | 8-10B | 16-20B |
| Flow Cell | Unit | Cycles | MGB | External Academic | External Industry |
| SP | Flow Cell | 100 | $2,892.00 | $3,965.00 | $4,378.00 |
| Flow Cell | 200 | $3,794.00 | $5,203.00 | $5,745.00 | |
| Flow Cell | 300 | $4,199.00 | $5,758.00 | $6,358.00 | |
| Flow Cell | 500 | $5,893.00 | $8,082.00 | $8,923.00 | |
| S1 | Flow Cell | 100 | $5,069.00 | $6,952.00 | $7,676.00 |
| Flow Cell | 200 | $6,407.00 | $8,787.00 | $9,702.00 | |
| Flow Cell | 300 | $6,998.00 | $9,597.00 | $10,597.00 | |
| S2 | Flow Cell | 100 | $9,349.00 | $12,822.00 | $14,157.00 |
| Flow Cell | 200 | $11,669.00 | $16,004.00 | $17,671.00 | |
| Flow Cell | 300 | $12,586.00 | $17,261.00 | $19,059.00 | |
| S4 | Flow Cell | 35 | $12,573.00 | $17,243.00 | $19,039.00 |
| Flow Cell | 200 | $16,959.00 | $23,258.00 | $25,681.00 | |
| Flow Cell | 300 | $18,947.00 | $25,984.00 | $28,691.00 | |
| NovaSeq Xp 2-Lane Kit | 1 kit | NA | $380.00 | $509.00 | $562.00 |
| NovaSeq Xp 4-Lane Kit | 1 kit | NA | $750.00 | $1,024.00 | $1,131.00 |
Miseq
| Flow Cell | Single Reads | Paired-End Reads |
| Nano v2 | 1M | 2M |
| Micor v2 | 4M | 8M |
| v2 | 12-15M | 24-30M |
| v3 | 22-25M | 44-50M |
| Flow Cell | Unit | Cycles | MGB | External Academic | External Industry |
| Nano | Flow Cell | 300 | $509.00 | $666.00 | $736.00 |
| Flow Cell | 500 | $606.00 | $794.00 | $876.00 | |
| Micro | Flow Cell | 300 | $713.00 | $933.00 | $1,030.00 |
| v2 | Flow Cell | 50 | $1,196.00 | $1,566.00 | $1,729.00 |
| Flow Cell | 300 | $1,466.00 | $2,011.00 | $2,220.00 | |
| Flow Cell | 500 | $1,658.00 | $2,274.00 | $2,511.00 | |
| v3 | Flow Cell | 150 | $1,283.00 | $1,759.00 | $1,942.00 |
| Flow Cell | 600 | $2,141.00 | $2,937.00 | $3,243.00 |
Library Preparation:
Generating high-quality data on the Illumina sequencing platform requires high-quality libraries which begin with your samples. Prices include initial sample QC, library preparation and final library QC analysis on the Tape Station and quantification by Qubit for each sample. Normalization concentration will depend on the end use.
| Service | Qty | Unit | MGB | External Academic | External Industry |
| NGS Library Preparation Only: | |||||
| Amplicon (180-240bp) | 1 | Sample | $71.50 | $98.00 | $109.00 |
| Long Amplicon (>400bp) | 1 | Sample | $74.10 | $102.00 | $113.00 |
| Whole Plasmid | 1 | Sample | $82.90 | $114.00 | $126.00 |
| NEBNext® Ultra™ II RNA Library Prep Kit for Illumina with poly(A) mRNA enrichment |
1 |
Sample | $109.60 | $151.00 | $166.00 |
| NEBNext® Ultra™ II RNA Library Prep Kit for Illumina |
1 |
Sample | $110.70 | $152.00 | $168.00 |
| NGS Library Preparation and Sequencing (minimum required) | |||||
| NGS GEDi Panel Sequencing (6 sample minimum) | 1 | Sample | $743.60 | $1,020.00 | $1,126.00 |
| Whole Genome Sequencing (24 sample minimum) | 1 | Sample | $ 900.00 | $1,291.00 | $1,426.00 |
| Post-Library QC -per sample (TapeStation and Qubit) | 1 | Sample | $18.00 | $25.00 | $27.00 |
Please read the following guidelines carefully to prevent delays in processing your NGS libraries.
| Application | Library Prep | Material required | Sample Volume | Insert Size | # Indexes Available |
| Amplicons | NEB Ultra II | 150ng-1ug | 20uL | 149-280bp | 480 |
| NEB Ultra II FS | 100ng-1ug | 20uL | >400bp | 480 | |
| WGS | KappaHyper Prep PCR free | 2-4ug | 50-100uL | NA | 96 |
| RNA | NEB Ultra II stranded, polyA or depletion method | 850ng | 50-100uL | NA | 480 |
| Plasmid | NEB Ultra II FS | 100-500ng | 20uL | 1-15kb | 96 |
| GEDi | Agilent SureSelect XT | 5ug | 100uL | NA | 96 |