In addition to Next-Generation Sequencing, Sanger sequencing, and array platforms and analyses, the OGI Genomics Core also offers the following services:
Next-Generation Sequencing Sample Preparation
SureSelect Targeted Enrichment, Agilent Technologies, Inc.
- CUSTOM panels/designs
Whole Genome, Illumina, Inc.
- TruSeq (large genomes)
- NEXTERA (small genomes)
Genomic DNA Blood Extraction
- Kit: Gentra PureGene Blood Kit
- Concentration/Purity assessment: NanoDrop 8000, Thermo Scientific, Inc.
- Certification: CLIA
Quantitative and Qualitative Biopolymer Analyses
NanoDrop 8000 (UV-Vis Spectrophotometer)
Up to eight simultaneous analyses
- Sample concentration
- Sample purity A260/A280 (protein) and A260/A230 (organics)
- Fluorescent labeling efficiency
Qubit Fluorometer, Life Technologies, Inc.
2200 TapeStation, Agilent Technologies, Inc.
Ultrasonication (i.e. “Mechanical Sheering”)
E220 Multisample Ultrasonicator, Covaris, Inc.
User-tuned for ultrasonication (i.e. “sheering”) of biological samples, including:
Can sequentially sheer between 1 – 24/48 samples
Bravo NGS workstation, Agilent Technologies, Inc.
Automated preparation of multiplexed, selective capture libraries for NGS